De Novo Assembly

De novo assembly is essential for analyzing gene expression in species lacking reference genomes, yet evaluating the accuracy of these assemblies remains a significant challenge. Fillmore and his collaborators developed RSEM-EVAL, a model-based scoring system designed to predict transcriptome assembly quality without requiring ground truth data. This methodology was validated through the assembly of the regenerating axolotl limb, providing a robust framework for improving the reliability of genetic data in non-model organisms.